Bd life sciences diagnostic systems qcpi manuals bd. For a limited time only, purchase a bd accuri c6 plus or a bd accuri c6 plus and csampler plus in combination with an extra year of warranty for a promotional price. All reagents and kits are compatible with both the bd accuri c6 plus and bd accuri c6 flow cytometer systems. Accuri c6 is a digital pc based bench top analyser, 2 laser system. Bd biosciences bd accuri c6 plus community, manuals and. Finally, the plant dna analysis template was opened. If you are interested in using a fluorochrome that is not listed here with the accuri c6, please contact the operator. Bd accuri c6 plus software features an intuitive user interface. Bd facscanto ii measuring instruments user manual manual pdf.
Cflow user guide 782001101 revd 1 1 introduction to cflow the accuri cflow or cflow plus software cflow allows you to control the c6 flow cytometer system to acquire data, generate statistics, and analyze results. If you have questions or require further clarification, see the bd accuri c6 flow cytometer instrument manual or contact accuri technical support. When setting up the cytometer, note the details in the following sections to maximize the instruments performance. Bd accuri c6 plus compensation calculator microsoft excel bd accuri c6 plus fluorochromedye reference chart. Accuri c6 maintenance cleaning and decontamination alternatives. For information on using bd accuri c6 software, see the bd accuri c6 software. Operate the instrument as described in this instruction manual. Bd accuri c6 software user guide manual 782009501 rev0 iii f.
Since yeasts are relatively large i use an accuri c6 to count bacteria so yeast are large to me i would go with the fasterlarger core size. The software for accuri c6 cflow plus is very userfriendly and efficient. After receiving instrument training from the flow cytometry facility staff, users are granted 247 access to the lab advanced booking is required. The system is equipped with blue 488 nm and red 640nm lasers, four variable fluorescence detectors and. Bd accuri c6 flow cytometers, bd cflow software and workstation pc supply all the capabilities of a fullfeatured flow cytometer at an affordable price. Bd accuri c6 compensation calculator microsoft excel bd accuri c6 flow cytometer optical filter guide user s guides and manuals. View online or download bd accuri c6 instrument manual. Figure 1a illustrates the overall standard cure conformity among the seven b. Bd accuri c6 flow cytometer instruction manual, serial number 2400 and higher.
If you are interested in using a fluorochrome that is not listed here with the accuri c6, please contact the operator click here to view the instrument manual. Deluca biochemical sciences building 440 henry mall madison, wi 53706. Bd accuri c6 flow cytometer instrument manual 7820018 rev4 1 1 introduction the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Accuri cytometers c6 flow cytometer community, manuals. Platforms referred to as bd accuri represent both the bd accuri c6 plus and bd accuri c6. The software for accuri c6 cflow plus is very user friendly and efficient. Acquired in 2015, the bd accuri system is a highquality, simple to use, flow cytometer capable of measurements of eukaryotic and prokaryotic cells. The facsverse is a far superior instrument to the c6.
Many bd accuri c6 customers can begin collecting and analyzing data with the help of a quick start guide. Bd accuri c6 plus panel builder users guides and manuals. Bd accuri c6 software controls the bd accuri c6 flow cytometer system in order. The accuri c6 flow cytometer does not produce aerosols during normal operation. Facsdiva v6 software overview course bd biosciences accuri c6 software user guide. Manual adjustment for gain on the laser exciting the reporter molecule was not possible for instruments a and f. Easytouse software with automated qc and application templates bd accuri c6 plus software features an intuitive user interface. Request a quote or demo on a bd accuri c6 plus system. For more detailed instructions on system operation details. Instrument includes a csampler, computer, software, validation report and warranty. We dont know what we are doing wrong, nor do we know how to fix it. Ill summarize the main conclusions here, for future reference for anyone dealing with similar issues. Accuri cytometers cflow user guide usf biological sciences. The intuitive interface of the software guides the user through workflows.
Comparison and evaluation of seven different bench. Bd accuri c6 flow cytometer quick set up guide please follow these directions for setting up your bd accuri c6 flow cytometer. Bd accuri c6 instrument manual instrument manual 31. The system includes blue and red lasers, four color detectors, and both forward and side scatter detectors plus software that is so intuitive that you will typically be up and running within. Always protect the sample introduction probe sip with a tube of hplc. Bd accuri c6 flow cytometer instrument manual 7820018 rev2 3 refer to the bd accuri c6 safety and limitations manual pn 7820091 for complete safety information. Even new flow cytometry users find it so easy to learn and use that. Bd serves and supports our customers with full offerings beyond our individual products, enabling integrated solutions across healthcare systems to comprehensively improve care and lower costs. Download user manual manual of bd facscanto ii laboratory equipment, measuring instruments for free or view it online on. Our neuroimmunology lab currently has a bd accuri c6 that has broken about every year and a half. I keep hearing that the bd accuri c6 flow cytometer is reliable.
Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Bd csampler software user guide iv 782009901 rev2 table of figures figure 11. Bd accuri c6 flow cytometer instrument manual bd biosciences. Bd accuri c6 flow cytometer quick set up guide umces. The bd accuri control software for the original bd accuri machines will install and run on both 32 and 64 bit windows versions. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Documentation bd accuri software user guide bd accuri user manual bd accuri user instructions installed in 2015. Performance validation of the bd accuri c6 was then done using 6 and 8peak fluorescent bead mixtures spherotech according to instructions bd accuri c6 software user guide. Ive recently started doing flow cytometry bd accuri c6 in a core facility at. The unique fluidics system of the bd accuri c6 plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Bd csampler software manual collect tab the following table describes each of the controls and indicators in the manual collect tab. The flow cytometry unit at university of helsinki integrates three flow cytometry facilities located at the meilahti and viikki campuses. Direct counts on the bd accuri c6 plus correlate highly with counting beads and are more precise than manual counts. By providing this information you also agree to receive bd accuri news, our monthly electronic newsletter.
The default settings display fsc and ssc data in linear scale and all fluorescence parameters in log scale. Data was generated on either the bd accuri c6 plus or the bd accuri c6 as indicated in figure legends. David kranzs lab click link for campus map is equipped with an accuri c6 4color flow cytometry analyzer. Bd accuri c6 software will display this notification based on whichever trigger is reached first. Bd csampler software user guide 782009901 rev0 23 figure 41.
However, the device driver is 32bit compatible only. Accuri cytometers c6 flow cytometer community, manuals and. Only operate the accuri c6 flow cytometer with an original ed version of software authorized by accuri. Accuri c6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to facscalibur. In addition, c6 has a flow metering system for automatic calculation of absolute cell counts. Bd accuri c6 flow cytometry system available for sale.
You can also set custom speedscore sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. Standard fluorochrome in use are identical to facs calibur. Accuric6 flow cytometry core facility university of bergen. Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. For information on installing and using the bd csampler, see the bd accuri. Accuri c6 flow cytometry analyzer the satelite facility at 348 burrill hall dr. The bd accrui c6 cytometer the cytometer has 2 lasersblue 488nm laser 3 colors. We have 1 bd accuri c6 manual available for free pdf download. Using the bd accuri c6 cytometer for rapid and accurate. Bd accuri c6 plus software files can be exported in fcs 3.
Discount is nontransferable and cannot be combined with other offers. Not for use in diagnostic or therapeutic procedures. Bd accuri c6 flow cytometer performance specifications summary tools. We offer stateofart analyzing and sorting flow cytometers for use to universityassociated and external researchers.
Please complete the following information to request a demonstration of the bd accuri c6 plus flow cytometer. View online user manual manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer. Assemble the computer according to the manufacturers instructions. Data was generated on either the bd accuri c6 plus or the bd accuri. Feb 20, 2017 bd accuri c6 flow cytometry system available for sale. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results. Bd accuri c6 instrument manual 31 pages flow cytometer. Promotional pricing for the standard bd accuri c6 plus flow cytometer or the standard bd accuri c6 plus flow cytometer with the bd csampler plus is only valid if one extra year of warranty is also purchased. Bd accuri c6 software will display this notification based on. It also includes common troubleshooting help and service information.
Bd accuri c6 flow cytometer instrument manual bd accuri c6 software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. Click here to view the bd fluorescence spectraviewer. Accuri cytometers c6 flow cytometer by accuri cytometers. Ive recently started doing flow cytometry bd accuri c6 in a core facility at my university, but my lab doesnt have any softtware to analyze the data. Accuri c6 maintenance cleaning and decontamination. Bd facscanto ii measuring instruments user manual manual. The bd accrui c6 cytometer university of washington. Install, update, and run antivirus protection software on a regular basis. Learn about bd life sciences diagnostic systems qcpi manuals. It is possible to measure some other fluorochromes using this setup. The accuri csampler is an optional accessory for the c6 flow cytometer. Accuri c6 is an easy to use flow cytometer which can be connected to an autosampler 48 and 96 well, and 24 tube rack, we do not have one.